Merck, CA200-1KT, cAMP Enzyme Immunoassay Kit, Direct

General description
Non-radioactive, competitive immunoassay for the quantitation of total cAMP in tissue and cell cultures treated with .1M HCL.The kit cAMP uses a cAMP polyclonal antibody to competitively bind the cAMP in the sample or alkaline phosphatase moleule that has cAMP covalently attachd to it.
Adenosine 3′,5′-cyclic monophosphate (cAMP) is a second messenger in many physiological processes. It is synthesized from adenosine triphosphate (ATP) in the presence of enzyme adenylate cyclase.
Application
Please refer to the attached Protocolfor details.
cAMP Enzyme Immunoassay Kit, Direct has been used to measure cAMP levels in cell culture medium virulent and attenuated macrophages. It has also been used to quantify cAMP synthesis in olfactory epithelium.
Biochem/physiol Actions
Adenosine 3′,5′-cyclic monophosphate (cAMP) is shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. Deciphering cAMP modulation in regulatory pathways may help in understanding of the physiology and pathology of many disease states.
Other Notes
Allow reagents to warm at room temperature for 30 minutes prior to opening.