Merck, CB_94031664, 215 BLCL human cell line

Biochem/physiol Actions
Not specified
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
Preparation Note
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
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HLA A: 1 2
HLA C: 7 0
HLA B: 7 8
HLA DR: 3 4
HLA DQ: 2 3
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Only the genomic DNA extracted from the cell line is available for purchase by commercial organizations, and not the cell line itself. No licensing agreement is required when the DNA extracted from this cell line are used for internal research purposes only. Frozen cell cultures can be supplied only to academic and not-for-profit organisations. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding other authorized use of this cell line or its genomic DNA may be directed to [email protected].
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.