Merck, CB1044-100UG, Anti-OCLN Mouse mAb (1G7)

General description
Purified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
Recognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.
This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.
Immunogen
A recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HL-60 cells, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)