Merck, CB1046-100UG, Anti-CLDN1 Mouse mAb (1C5-D9)

General description
Purified mouse monoclonal antibody. Recognizes the ~17 kDa CLDN1 protein.
Recognizes the ~17 kDa CLDN1 protein in PRC/PRF/5 and A431 cells and human small intestine and colon tissue.
This Anti-CLDN1 Mouse mAb (1C5-D9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of CLDN1.
Immunogen
Full-length, recombinant, human CLDN1 (aa 1-212), expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3-5 µg/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
PRC/PRF/5 cells, A431 cells, Human small intestine tissue, human colon tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Guevin C, et al. 2009. Antiviral Res.84, 14.
Sainz, B., Jr, et al. 2009. Virol. J.6, 103.
Stamataki Z, et al. 2009. Blood113 585.
Farquhar MJ, et al. 2008. J. Virol.82 8797.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)