Merck, CBL458F, Anti-CD49f Antibody, FITC conjugated, clone 4F10, FITC conjugated

Immunogen
Cell line SW/222 (Human colorectal carcinoma).
Application
Anti-CD49f Antibody, FITC conjugated, clone 4F10, FITC conjugated is an antibody against CD49fd for use in FC, IP, IH.
Immunohistological studies on frozen sections

Immunoprecipitation of the laminin receptor

Flow Cytometry: 10 μL per 10⁶

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Biochem/physiol Actions
This antibody is specific to CD49f (integrin alpha6) chain) which is expressed on epithelial and endothelial basement membrane. The VLA alpha6 chain, which consists of a single disulfide linked 120 kDa subunit and a small doublet subunit of 25/30 kDa, is noncovalently-associated with the VLA beta-chain (CD29) in VLA-6 or alpha6/beta1 complex or with integrin beta4 chain (alpha6/beta4). The antigen is expressed on platelets, monocytes, thymocytes and T lymphocytes.

FUSION PARTNER: NS1 myeloma cell line
Physical form
Protein A purified immunoglobulin conjugated to fluorescein isothiocyanate (FITC). Liquid in PBS containing 1mg/mL BSA and 10mM sodium azide.
Preparation Note
Maintain at 2-8°C for up to 12 months from date of receipt, protected from light. DO NOT FREEZE.
Other Notes
Replaces: MAB2254F
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.