Merck, CBL517, Anti-CD28 Antibody, clone 15E8

Immunogen
Human T lymphocytes
Application
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-CD28 Antibody, clone 15E8 is validated for use in FC, FUNC for the detection of CD28.
To study the role of CD28 in T cell proliferation. The antibody will induce the proliferation of resting T lymphocytes.

This antibody will act in cell stimulation studies where it has been suggested that measuring T cell function in HIV infected asymptomatic patients may be an early indicator of AIDS, independent of CD4 + cell counts

Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
This antibody reacts with the 44 kDa antigen found on a sub-population of T cells and activated B cells. These CD28+ CD8 cells are now known to be cytotoxic T cell precursors. The antibody does not react with B cells, granulocytes or monocytes. The ligand for CD28 is CD80 (B7) which is found on activated B cells suggesting a role for CD28 in T and B cell interaction

FUSION PARTNER: SP2/0
Physical form
Format: Purified
Please see lot specific datasheet for product formulation. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Preparation Note
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL517P
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.