Merck, CLS354232-1EA, Corning®Laminin

General description
Corning Laminin, mouse, 1mg, is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.
Quality
Purity >90% by SDS-PAGE (contains entactin)
Tested for ability to initiate neurite outgrowth of NG-108 human neuroblastoma cells
Found negative for bacteria, fungi, and mycoplasma

Source
Engelbreth-Holm-Swarm (EHS) mouse tumor

Formulation
Frozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4

Molecular Weight
900 kD (unreduced)

Preparation and Storage
Stable for at least three months at -70°C.

Handling
Recommended concentration: 1-10 μg/cm² of growth surface depending on cell type
Legal Information
Corning is a registered trademark of Corning, Inc.