Merck, CLS356008-1EA, Corning®Fibronectin

General description
Corning Fibronectin, human, 5mg, is used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types. The principal functions of fibronectin appear to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.
Quality
Purity = 90% by SDS-PAGE
Tested for ability to promote attachment and spreading of BHK-21 cells
Found negative for bacteria, fungi, and mycoplasma

Source
Human plasma. Source material tested for hepatitis B antigen and HIV-1 antibody.

Molecular Weight
440 kD (unreduced form)

Preparation and Storage
Stable for three months at 2° to 8°C (lyophilized) or two weeks at -20°C (solubilized)

Handling
Recommended concentration - 1-5 μg/cm² of growth surface for coating or at 5 μg/ml as a media additive

Formulation
Lyophilized from CAPS buffer containing NaCl and CaCl2, pH 11.0
Legal Information
Corning is a registered trademark of Corning, Inc.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.