Description
General description
This kit includes pooled virus representing each of the 2 possible capture sequences and 2 position combinations (capture sequences 1 or 2, in the stem-loop or 3 position). Present within each pool are 2 CRISPRi sgRNAs, 1 targeting RAB1A and 1 non-targeting control sgRNA. Each pool is provided ready to use at 1x106 TU/mL, sufficient enough to cover 2x106 cells at an MOI of 0.5. The purpose of the optimization kit is to set up a pilot screen to determine the best capture sequence and position configuration before a custom pool is ordered.
We have an exclusive partnership with 10x Genomics & our compatible CRISPRi pools are a perfect complementary product for target gene validation following a whole genome screen.
The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools. For more information about single cell screening with CRISPR and 10x genomics Click Here.
Application
Optimization kit for 10x CRISPRi screening
Pooled CRISPR Screening with 10x Genomics Compatibility
Strong transcriptional inhibition in multiple cell lines
Creation of cell lines stably expressing KRAB-dCas9
Validated positive and negative controls
Biochem/physiol Actions
CRISPR lentiviral products are powerful research tools for discovering novel genes and cellular pathways essential to understanding human health and disease. As a world leader in lentiviral libraries′ production, we design and build custom pools to any specifications. We have partnered with 10x Genomics to bring innovative pooled screening products to enable discovery at single-cell resolution with this capability.
CRISPR-based functional genomics screening provides a broad overview of the genetic contributions to a measurable phenotype. Bulk screening, however, only provides limited functional information beyond the selectable phenotype. At the other end of the spectrum, the single-cell analysis provides high content information centered around genome-wide transcriptome profiling for candidate genes. We offer optimized, customized lentiviral vectors compatible with Chromium Single Cell Gene Expression utilizing Feature Barcode technology.
Features and Benefits
Focus on your research, and we will generate your lentivirus screening library
No need for a proxy barcode or risk of recombination
Resolve complex gene regulatory networks critical for biomarker or drug target discovery
Analysis of single-cell perturbations from a mixed population reduces the noise in pooled screens and allows for whole transcriptome interrogation of rare cells
Simplicity: 10x Feature Barcode technology compatible lentivirus CRISPR pool
Flexibility: Submit your gRNA pool or have our team select gRNAs for you Focus on your research, and we will generate your lentivirus screening library
Other Notes
4 Subpools with a minimum concentration of 1x106 TU/ml (via FACS assay)
CRISPRI02: RAB1A And Nontargeting Control With 10x Capture Sequence 1 Stem-Loop Position
CRISPRI03: RAB1A And Nontargeting Control With 10x Capture Sequence 1 3 Prime Position
CRISPRI04: RAB1A And Nontargeting Control With 10x Capture Sequence 2 Stem-Loop Position
CRISPRI05: RAB1A And Nontargeting Control With 10x Capture Sequence 2 3 Prime Position
1 Effector Construct with a minimum concentration of 1x105 TU/mL (via FACS or CFU assay)
CRISPRIE: KRAB-dCAS9 CRISPRi Construct Lentiviral Transduction Particles
Legal Information
CRISPR Use License Agreement
Lentiviral, WPRE and Evrogen Fluorophore Licenses
