Merck, CS0170-1KT, Thioredoxin Reductase Assay Kit

General description
The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H⁺ ↔ 2 TNB + NADP⁺
Application
Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).
Features and Benefits
An easy and simple method
The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells