Merck, CSA001-1, Rho Kinase (ROCK) Activity Assay

The Millipore Rho-associated kinase activity assay is a plate-based biochemical test for measuring activity associated with ROCK and related DMPK-family kinases in suitable research samples. The assay family uses an MYPT1-coated 96-well plate and detects phosphorylation of MYPT1 at Thr696 through a specific primary antibody and horseradish peroxidase-linked secondary antibody. A chromogenic TMB readout can then be measured in a compatible microplate reader, with substrate and inhibitor controls helping define the assay response. The complete assay workflow requires carefully prepared lysates or kinase preparations, ATP/Mg²⁺ conditions, timed incubation, washing and appropriate normalization to protein input. A phosphorylation signal is not automatically specific for ROCK under every sample condition because related kinases and endogenous phosphatases can influence the result. Include negative, inhibition and reference controls when comparing cell treatments. The named product is discontinued, so any use of existing stock must be preceded by assessment of expiration, reagent storage and the appropriate archived instructions rather than assumptions about current availability.