Merck, D5652-50L, Dulbecco′s Phosphate Buffered Saline

General description
Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
Application
D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
to dissolve Angelica gigas powder for microencapsulation
to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
to wash amniotic membrane for cellular analyses

Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Other Notes
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available