Merck, D9156-5X1ML, Deoxyribonucleic acid, single stranded from salmon testes

Name: Deoxyribonucleic acid, single stranded from salmon testes
Brand: Merck
SKU: D9156-5X1ML

Size:5 x 1 mL, single-stranded template DNA, For hybridization

Available Catalog Numbers

D9156-1ML D9156-5ML D9156-5X1ML

Catalog Number: D9156-5X1ML

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Category

Nucleotides & Nucleosides

Pack Size5 x 1 mL

TagsChemistry & Biochemicals Biochemicals Nucleosides & Nucleotides

CollectionsMerck Biochemicals Collection: Trusted Lab Reagents & Buffers Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .
Application
Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples and human blood monocyte subpopulations.
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Preparation Note
This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

Specifications

General

UNSPSC Code	41106310
eCl@ss	32160414
NACRES	NA.52
MDL number	MFCD00130921
biological source	fish testis (salmon)
grade	Molecular Biology
assay	9-11 mg/mL (DNA concentration)
form	solution
concentration	10 mg/mL in H2O
shipped in	dry ice
storage temp.	−20°C
Quality Level	200

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