Merck, DR1025-50UL, PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb

General description
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser³¹⁷.
Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser³¹⁷ in UV-treated COS cells.
This PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).
Immunogen
Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1
Application

Immunoblotting (1:1000)
Immunocytochemistry (1:500, fluorescence)
Paraffin Sections (1:50)
Physical form
In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Preparation Note
Do not aliquot.
Analysis Note
Positive Control
Lysates from COS cells treated with UV
Other Notes
Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.
Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.
Shieh, S.Y., et al. 2000. Genes Dev.14, 289.
Martinho, R.G., et al. 1998. EMBO J.17, 7239.
Zeng, Y., et al. 1998. Nature395, 507.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)