Merck, DR1047-100UG, Anti-UHRF1 Mouse mAb (3A11)

General description
Purified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.
Recognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.
This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.
Immunogen
A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
NA
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, Human endometrium tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Mistry H, et al. 2008. Biochem. Biophys. Res. Commun.375 321.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)