Merck, DR1061-100UG, Anti-ENO1 Mouse mAb (8G8)

General description
Anti-ENO1, mouse monoclonal, clone 8G8, recognizes the ~45-48 kDa ENO1 in MCF-7 and HeLa cells and in human lymphoma tissue. It is validated for use in ELISA, WB, ICC, & IHC (paraffin sections).
Purified mouse monoclonal antibody. Recognizes the ~45-48 kDa ENO1 protein.
Recognizes the ~45-48 kDa ENO1 protein in MCF-7 and HeLa cells and human lymphoma tissue.
Immunogen
Full-legth, recombinant, human, ENO1 (aa1-435), expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (1 g/ml)
Physical form
In PBS, pH 7.30.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
MCF-7 cells, Human lymphoma tissue, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Ho, J.A., et al. 2010. Anal. Chem.82 5944.
Zhao, J., et al. 2007. Mol. Cell Proteomics.6 987.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)