Merck, DR1075-100UG, Anti-TARDBP Mouse mAb (2E2-D3)

General description
Purified mouse monoclonal antibody. Recognizes the ~45 kDa TARDBP protein.
Recognizes the ~45 kDa TARDBP protein in A431 and HeLa cells and human leiomyosarcoma tissue.
This Anti-TARDBP Mouse mAb (2E2-D3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TARDBP.
Immunogen
Full-length, recombinant human TARDBP (aa 1-261), expressed as a GST fusion protein.
Application

Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (5 µg/ml)
Physical form
In PBS, pH 7.7.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
Positive Control
A431 cells, Human leiomyosarcoma tissue, HeLa cells
Other Notes
Ilieva, E,V., et al. 2010. Free Radic. Biol. Med.48, 1302.
Wils H, et al. 2010. Proc. Natl. Acad. Sci. U S A107 3858.
Foulds, P,G., et al. 2009. Acta Neuropathol.118 647.
Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)