Merck, DR1094-100UG, Anti-PAX7 Mouse mAb (1E12)

General description
Purified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
Recognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.
This Anti-PAX7 Mouse mAb (1E12) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PAX7.
Immunogen
A recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
NIH3T3 cells, Human stomach tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)