Description
Application
Specificity
Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells are required.
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Features and Benefits
Multicolor Duolink® proximity ligation assay (PLA®) is an application of the Duolink® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink® PLA Probemaker Guide for Multicolor Detection and the Duolink® PLA Multicolor Detection protocol to use this product.
Visualize and detect multiple proteins events in one experiment.
No overexpression or genetic manipulation required
High specificity (fewer false positives)
Detection of low abundant proteins due to rolling circle amplification
Relative quantification possible
No special equipment needed
Faster and more simple than FRET
Increased accuracy compared to co-IP
Publication-ready results
Components
Duolink® PLA Multicolor Probemaker Kit - Red contains all the necessary reagents to perform the conjugations of Red Oligo A and Red Oligo B directly to primary antibodies. The resulting PLA probes (Red A and Red B) must be used together and cannot be paired with any other Duolink
This product is comprised of the following:
Red Oligo A DUO86010A
Red Oligo B DUO86010B
Conjugation Buffer DUO82033
Stop Reagent DUO82034
Storage Solution DUO82035
See datasheet for more information.
To perform a Multicolor Duolink® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink® Wash Buffers and Mounting Medium.
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies
