Merck, E2264-.2UN, Endoglycosidase F3 from Elizabethkingia miricola

Application
Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.
Biochem/physiol Actions
Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.
Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.
Packaging
Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5
Physical form
Aseptically filled solution in 20 mM Tris-HCl, pH 7.5
Other Notes
One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.