Merck, E8022-20UG, pRc/RSV Expression vector

General description
pRc and pRSV Supercoiled Expression Vectors (5.2 kb) are for protein expression in mammalian cells. Each vector contains:
The polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
The SV40 replication origin for episomal replication
The f1 origin for rescue of the sense strand
The neomycin resistance gene, and
An ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.

The primary difference between these expression vectors is that pRc contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and pRSV from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.

The pRc MCS contains unique ApaI, HindIII, NotI, and XbaI restriction sites, plus redundant BstXI sites.

The pRSV MCS contains unique HindIII, NotI, SpeI, and XbaI restriction sites, plus redundant BstXI sites.
Biochem/physiol Actions
The MCS contains unique ApaI (CMV2 only), HindIII, NotI, SpeI (RSV only), and XbaI restriction sites, plus redundant BstXI sites.
Features and Benefits
Supercoiled vectors for protein expression in mammalian cells. Both of these vectors contain:
the polyadenylation signal and transcription termination sequence from BGH to enhance mRNA stability
the SV40 replication origin for episomal replication
the f1 origin for rescue of the sense strand
the neomycin resistance gene, and
an ampicillin resistance gene and colE1 origin for selection and maintenance in E. coli.
The primary difference between these expression vectors is that one contains the enhancer-promoter sequence from human cytomegalovirus (CMV2) and the other from Rous sarcoma virus (RSV). Both are strong promoters for high-level transcription.