Merck, E9402-200UG, EphB2/Fc Chimera from mouse

Name: EphB2/Fc Chimera from mouse
Brand: Merck
SKU: E9402-200UG

Size:200 μg, Cek5, Drt, ERK, Hek5, Nuk, Qek2, Sek3, Tyro5, >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Catalog Number: E9402-200UG

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Category

Cell-Free Protein Expression Kits

Pack Size200 µg

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
EphB2 (Epherin receptor B2) is a receptor tyrosine kinase (RTK), which belongs to a 13 member family of ephrin receptors. This family is divided into two classes A and B. EphB2 belongs to EphB group, which contains five members. The ligands to this group of receptors are named ephrin-B1-B3. It is a bidirectional functioning membrane protein.
Application
EphB2/Fc Chimera from mouse has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain.
Biochem/physiol Actions
EphB2 (Epherin receptor B2) is involved in the development and plasticity of glutamatergic synapses, and their interaction with NMDA receptor. It can also regulate the processing of acute inflammatory pain, and mechanical allodynia as a result of nerve crush. Activation of this receptor results in recruitment and activation of Src-protein kinases. These, in turn, phosphorylate and activate NMDA receptor. It regulates the function of platelets and the development of thrombus. It is also responsible for the retraction of clots, and modulates independent platelet function in a contact-independent manner. EphB2 signaling is also involved in the modulation of axonal sprouting, which is induced by lesions.
Member of the Eph receptor tyrosine kinase family shown to bind ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis, and in arterial/venous demarcation of the developing vasculature.
Physical form
Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0.
Analysis Note
The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay.
Other Notes
Extracellular domain of mouse EphB2 (amino acids 1-548) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.

Specifications

General

UNSPSC Code	51111800
NACRES	NA.32
MDL number	MFCD01867526
biological source	mouse
recombinant	expressed in NSO cells
assay	>90% (SDS-PAGE)
form	lyophilized powder
potency	0.04-2.5 ng/mL
mol wt	monomer calculated mol wt 85.3 kDa
packaging	pkg of 200 μg
storage condition	avoid repeated freeze/thaw cycles
technique(s)	ligand binding assay: suitable
impurities	endotoxin, tested
UniProt accession no.	P54763
storage temp.	−20°C
Quality Level	100
Gene Information	mouse ... Ephb2(13844)

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Merck, E9402-200UG, EphB2/Fc Chimera from mouse

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