Merck, E9762-1UN, Endoglycosidase F1 from Elizabethkingia miricola

General description
Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.
Application
Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.
Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.
Biochem/physiol Actions
Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.
Packaging
Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.
Physical form
Aseptically filled solution in 20 mM Tris-HCl pH 7.5
Other Notes
One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.