Merck, F0628-5UN, Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)

Name: Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)
Brand: Merck
SKU: F0628-5UN

Size:5 units, Ferridoxin-NADP⁺ oxidoreductase, lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

Available Catalog Numbers

F0628-10UN F0628-1UN F0628-5UN

Catalog Number: F0628-5UN

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Category

Vacuum Filtration Units

Pack Size5 units

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Ferredoxin-NADP⁺ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.
Application
Ferredoxin-NADP⁺ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.
Ferredoxin-NADP⁺ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.
Biochem/physiol Actions
Ferredoxin-NADP⁺ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Ferredoxin-NADP⁺ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Other Notes
One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.

Specifications

General

Enzyme Commission (EC) Number	1.18.1.2 ( BRENDA \| IUBMB )
UNSPSC Code	12352204
NACRES	NA.54
MDL number	MFCD00131050
form	lyophilized powder
specific activity	≥15 units/mg solid
foreign activity	NADH diaphorase activity ≤10 units/mg solid
storage temp.	−20°C
Quality Level	200

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