Description
General description
Goat polyclonal anti-Guinea Pig IgG (whole molecule)−FITC antibody reacts with guinea pig IgG (whole molecule). Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Anti-Guinea Pig IgG (whole molecule)-FITC antibody is specific for guinea pig IgG subclasses. Goat anti-Mouse IgG is purified by affinity isolation and conjugated to FITC.
Second antibodies (secondary antibodies) are antibodies raised against other antibodies, typically from another species, called primary antibodies. Primary antibodies are generated with and bind to specific antigens to create antigen antibody complexes which may be detected in a variety of immunochemical and immunohistochemical configurations involving soluble or insoluble signaling molecules. The immunodetection system depends upon the binding of a signal competent secondary antibody to a primary antibody:antigen complex.
The suitability of any secondary antibody to a specific immunodetection application is dependent upon its signal development system configuration. Signal development systems are based upon the chemical conjugation of a signal mediator to the secondary antibody. Major secondary antibody signal systems are color (chromogenic), chemiluminescence or fluorescence based and involve the use of enzymes or high affinity binary systems such as biotin:avidin.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Immunogen
Purified guinea pig IgG
Application
Anti-guinea pig IgG (whole molecule)-FITC antibody may be used for immunofluorescence of guinea pig spleen cells at a minimum dilution of 1:64. Antibody concentrations ranging from 1:80-1:100 were used for immunofluorescence in various studies. Guinea pig IgG was used as secondary antibody to quantitate fluorescence by photon counting using a Leitz MPV Compact 2 microscope photometer.
Goat polyclonal anti-Guinea Pig IgG (whole molecule)−FITC antibody may be used as a secondary antibody or to detect the presence of guinea pig IgG via fluorogenic immunochemical or immunohistochemical techniques.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
