Merck, F6797-500G, Frey mycoplasma broth base

Application
Used for cultivation of Avian Mycoplasma.
Frey mycoplasma broth base may be used as a culture medium for examining various proteins of Mycoplasma pulmonis.
Preparation Note
Suspend 22.3 g of Frey Mycoplasma Broth Base in 900 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add 100 ml of sterile heat inactivated horse serum (H1138). Mix well before dispensing.
Other Notes
Ingredients (g/L)
Casein enzymic hydrolysate, 7.50
Papaic digest of soyabean meal, 2.50
Yeast extract, 5.00
Sodium chloride, 5.00
Potassium chloride, 0.40
Magnesium sulphate, 0.20
Disodium phosphate, 1.60
Monopotassium phosphate, 0.10
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany