Description
General description
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples.
Application
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi. It has also been used to measure the ATP content in oocytes and harderian gland(HG) tissue homogenates.
Preparation Note
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO₄, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts.
Reconstitute with 50 mL of sterile water.