Merck, G7509-10UN, GABase from Pseudomonas fluorescens

Name: GABase from Pseudomonas fluorescens
Brand: Merck
SKU: G7509-10UN

Size:10 units, GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase, lyophilized powder, Protein ≥30 % by biuret

Available Catalog Numbers

G7509-10UN G7509-25UN

Catalog Number: G7509-10UN

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Category

Recombinant Enzymes

Pack Size10 units

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Research area: Neuroscience

GABase is a commercially available mixture composed of γ-aminobutyric acid aminotransferase (GABGT) and succinic semialdehyde dehydrogenase (SSDH) obtained from Pseudomonas fluorescens.
Application
GABase from Pseudomonas fluorescens has been used for GABase assay to quantify the extracellular and intracellular concentration of GABA (γ-aminobutyric acid).
Biochem/physiol Actions
GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.
Physical form
Partially purified lyophilized powder containing buffer salts and stabilizer
Other Notes
One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.

Specifications

General

UNSPSC Code	12352204
NACRES	NA.32
MDL number	MFCD00131113
biological source	Pseudomonas fluorescens
assay	≥30 % (biuret)
form	lyophilized powder
specific activity	≥0.5 units/mg protein
composition	Protein, ≥30% biuret
technique(s)	inhibition assay: suitable
storage temp.	−20°C
Quality Level	200

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