Merck, GE17549854, HiTrap®MabSelect™PrismA

General description
These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand, allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met, including processing of many bispecific antibodies.

The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes, which can be exposed to days or weeks of feed harvest.
Features and Benefits
Enhanced binding capacity compared with other Protein A resins
Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH
Covered by a comprehensive security of supply program, including dual sources of the agarose base matrix and protein A ligand.
The PrismA protein A ligand is also available in HiTrap® Fibro and HiScreen™ Fibro PrismA chromatography units for ultrafast purification of mAbs.
Easily measure protein A leakage using the PrismA ELISA kit specifically designed for use with MabSelect™ PrismA resins.

Legal Information
HiScreen is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva