General descriptionCyDye™ DIGE Fluor minimal dyes and saturation dyes are size- and charge-matched fluorescent dyes specifically designed for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis using Ettan™ DIGE system.
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection. In total, three minimal dyes and two saturation dyes are available.
CyDye™ DIGE fluors are exceptional dyes for multicolor analysis, offering bright and intense colors with narrow excitation and emission bands. The fluors are spectrally distinct, making them highly suitable for multicolor detection. CyDye™ DIGE fluors utilize these benefits but are also size- and charge-matched specifically for 2-D DIGE using Ettan™ DIGE system.
Protein samples and the internal standard are each labeled with one CyDye™ DIGE Fluor minimal dye. These labeled samples are then combined, run on an isoelectric focusing gel in the first dimension, and separated by SDS-PAGE in the second dimension. Electrophoresis is simplified with Ettan™ IPGphor 3, or Multiphor II with Immobiline® DryStrip gels in the first dimension and Ettan™ DALTtwelve or Ettan™ DALTsix electrophoresis systems in the second dimension.
The ability to multiplex different CyDye™ DIGE Fluor minimal dye-labeled samples on the same gel means that the different samples will be subjected to exactly the same first- and second-dimension running conditions. Consequently, the same protein labeled with any of the CyDye™ DIGE Fluor minimal dyes and separated on the same gel will migrate to the same position on the 2-D gel and overlay. This limits experimental variation and ensures accurate within-gel matching.
Features and BenefitsAllow detection of up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel.
Size- and charge-matched dyes enable co-migration of labeled samples within the gel.
Bright and highly sensitive dyes allow the use of the minimal labeling technique.
Minimal loss of signal during labeling, separation, and scanning.
No change in signal over wide pH range used during first-dimension (IEF) separation.
Discrete signal from each fluor with minimal cross-talk contributes to high accuracy.
Preparation NotePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis NoteTo view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal InformationCyDye:
Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.
CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva.
CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.
CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646.
Cy and CyDye, are - trademarks of Cytiva or one of its subsidiaries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes. A license to use the Cy and CyDye trademarks for commercial purposes is subject to a separate license agreement with Cytiva. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug screening.
If you require a commercial license to use the Cy and CyDye trademarks please contact
[email protected].
If you require a commercial license to use this material and do not have one, return this material unopened to Cytiva Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the material will be refunded.
Cy is a registered trademark of Cytiva
CyDye is a trademark of Cytiva
Ettan is a trademark of Cytiva
Immobiline is a registered trademark of Cytiva
