Merck, GE28-4017-48, GSTrap™4B

Name: GSTrap™4B
Brand: Merck
SKU: GE28-4017-48

Size:1 pkg, GSTrap Column, Cytiva 28-4017-48, pack of 5 × 5 mL

Available Catalog Numbers

GE28-4017-45 GE28-4017-47 GE28-4017-48

Catalog Number: GE28-4017-48

Pricing

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Category

Protein Purification Columns

Pack Sizepack of 5

TagsLabware Labware Partners Cytiva™ Products

CollectionsMerck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
GSTrap™ 4B columns are prepacked Glutathione Sepharose™ 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Application
GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap™ 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose™ 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC™ System.
Features and Benefits
High binding capacity.
Mild elution conditions preserving Protein antigenicity and function.
Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
GSTrap™ 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.

Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Specifications

General

NACRES	NA.56
UNSPSC Code	23151816
ligand	glutathione
packaging	pack of 5 × 5 mL
manufacturer/tradename	Cytiva 28-4017-48
parameter	<20 mL/min flow rate
bed size	16 mm × 25 mm
bed volume	5 mL
column I.D.	16 mm
matrix	4% agarose
particle size	45-165 μm
average diameter	90 μm
cleaning	4-13
working range	4-13
capacity	>25 mg binding capacity (horse liver GST/ml medium)

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

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