Description
General description
HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Application
HisTrap HP columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.
Features and Benefits
High binding capacity, at least 40 mg/ml chromatography medium.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Negligible Ni²⁺leakage
Simple manual operation with a syringe, pump, or chromatography system such as AKTA design
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Legal Information
HisTrap is a trademark of Cytiva