General description
Cy3™ Maleimide Mono-Reactive Dye 5-Pack
More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions.
Provides an alternative when CyDye™ NHS Esters are unsuitable.
Dyes are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.
Cy3™ and Cy5 mono-functional maleimides are particularly suitable for the selective labeling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides. They are also useful in antibody labeling since the dye molecules are introduced away from the antibody binding site, increasing the chance that functionality of the labeled molecule will be unimpaired.
The protocol is simple and robust, offering an alternative chemistry when either greater selectivity of labeling is required or amine labeling with Cy3™ and Cy5 NHS Esters is not desirable or appropriate.
Components Each kit contains five vials of dye, each sufficient for labeling 1 mg of protein.
Lifetime:0.15
Molar Extinction Coefficient:150000 M-1 cm-1
Color:Red
Storage Conditions:2 - 8° C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20° C in aliquots to avoid repeat freeze thaw cycles.
Application
For the labelling of biological compounds with Cy3™ monofunctional dye.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
CyDye:
Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries.
CyQ (or Cy5Q or Cy7Q): These products are covered under US patent number 6,828,116 and equivalent patents and patent applications in other countries in the name of Cytiva.
CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva.
CypHer5E: This product or portions thereof is manufactured under license from Carnegie Mellon University and is covered by US patent number 7,615,646.
Cy and CyDye, are - trademarks of Cytiva or one of its subsidiaries.
The purchase of CyDye products includes a limited license to use the CyDye products for internal research and development but not for any commercial purposes. A license to use the Cy and CyDye trademarks for commercial purposes is subject to a separate license agreement with Cytiva. Commercial use shall include:
1. Sale, lease, license or other transfer of the material or any material derived or produced from it.
2. Sale, lease, license or other grant of rights to use this material or any material derived or produced from it.
3. Use of this material to perform services for a fee for third parties, including contract research and drug screening.
If you require a commercial license to use the Cy and CyDye trademarks please contact
[email protected].
If you require a commercial license to use this material and do not have one, return this material unopened to Cytiva Bio-Sciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden and any money paid for the material will be refunded.
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva
CyDye is a trademark of Cytiva
