Description
General description
EIAs and ELISAs are convenient, high-sensitivity, peroxidase-based technologies that enable the amount of protein present in a sample to be measured in a single day. Novel lysis reagents have been developed (Lysis Reagents 1 and 2) to facilitate simple and rapid extraction of cellular molecules.
The Biotrak™ cGMP competitive enzymeimmunoassay system is specifically designed for research purposes. The kit includes protocols using novel lysis reagents in order to facilitate simple and rapid extraction of cGMP from cell cultures. These components avoid the requirement for traditional, time-consuming extraction procedures and obviate the need for removal of extraction reagents prior to measurement. It combines the use of a peroxidase-labeled cGMP conjugate, a specific antiserum which can be immobilized on to precoated microplates, and a one-pot stabilized substrate solution.
Lysis reagent 1 hydrolyzes cell membranes to release intra-cellular cAMP. Lysis Reagent 2 sequesters the key component in Lysis Reagent 1 and ensures cAMP is free for subsequent analysis. The detergent/sequestrant complex does not interfere with antigen: antibody binding. Lysis Reagent 1 is simply added to cultured cells, followed by a 5 to 10 min incubation before assay. The antiserum is reconstituted with Lysis Reagent 2. The assay is based on competition between unlabeled cAMP and a fixed quantity of peroxidase-labeled cAMP, for a limited number of binding sites on a cAMP specific antibody.
Application
cGMP, Biotrak™ EIA System has been used for measuring total cellular cyclic GMP (cGMP) or cyclic AMP (cAMP) levels.
Features and Benefits
Elimination of inconvenient, time-consuming extraction procedures.
Flexible method-choice of assay protocols.
Dual range 2–512 fmol/well (acetylation protocol), dual range 50–12 800 fmol/well (non-acetylation protocol).
Rapid assay protocol.
Nonradioactive.
Specific for cGMP
Precise and accurate measurement.
Ready-to-use substrate.
Colour coded reagents
Preparation Note
Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Biotrak is a trademark of Cytiva
