Merck, GF06, Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3)

General description
Recognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa denatured and native forms of TGFα protein.
Immunogen
recombinant, human TGF-α (~6 kDa mature form)
Human
Application
Immunoblotting (1-5 µg/ml, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Preparation Note
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
Analysis Note
Negative Control
NIH3T3 cells
Positive Control
A2058 or ST-FeSV FRE cells
Other Notes
Sorvillo, J.M., McCormack, E.S. 1990. Oncogene5, 377.
Coffey, R.J., et al. 1987. Nature328, 817.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
This antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)