Description
General description
Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGFβ superfamily. It plays a dominant role in embryonic dorsalventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant human BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.
Product Source: E. coli.
Application
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Biochem/physiol Actions
Cross Reactivty
Mouse, Rat, Rabbit, Cow, Dog, Monkey
Physical form
Product is filtered through a 0.2 micron filter before lyophilization.
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Bioactivity assay: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
