Merck, GF180, MIF,Human Recombinant

General description
Macrophage Migration Inhibitory Factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-a, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue.
Insect cell derived recombinant MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
Product Source: Protein is expressed in insect cells.
Application
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Physical form
Product is presented in 10mM Sodium Citrate, pH 3.0 and is filtered through a 0.2 micron filter before lyophilization.
Preparation Note
Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Analysis Note
Determined by its ability to inhibit monocyte migration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.