Merck, GF318, BAFF, Human Recombinant Animal Free

General description
BAFF, a member of the TNF superfamily of ligands, is expressed in T cells, macrophages, monocytes and
dendritic cells. BAFF is involved in stimulation of B and T cell function, and is an important survival and maturation factor for peripheral B cells. BAFF signals through three different TNF receptors TACI, BCMA and BAFF-R.
Recombinant human soluble BAFF is a 152 amino acid polypeptide (17.0 kDa), which contains the TNF-like portion of the extracellular domain of BAFF.
Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.
Application
Research Category
Stem Cell Research
Research Sub Category
Growth Factors & Receptors
Physical form
Product is presented in 20 mM Tris, pH 8.0 + 215mM NaCl and is filtered through a 0.2 micron filter before lyophilization.
Preparation Note
Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.
Analysis Note
Determined by dose-dependent NFκB activation in HEK293 BCMA–NFκB reporter cells.
The expected ED50 for this effect is 100-200ng/ml.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.