Merck, GF444, HumanKine® VEGF121, Human Recombinant Xeno-Free

General description
HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
Vascular endothelial growth factor (VEGF) is active in angiogenesis, vasculogenesis and endothelial cell growth. VEGF induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. VEGF binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. VEGF121 is acidic and is a freely secreted form of VEGF.
Physical form
Product is presented in PBS and filtered through a 0.2 micron filter before lyophilization.
Preparation Note
Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.
Analysis Note
Bioactivity assay: Determined by the dose-dependent stimulation of the proliferationof HUVEC cells (Human Umbilical Vein
Endothelial Cells). ED50 is typically 2-8 ng/ml.
HumanKine VEGF121 is manufactured using all non-animal reagents and is considered Xeno-Free
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.