Merck, GR13L-100UG, Anti-EGFR (Ab-3) Mouse mAb (225)

General description
Anti-EGFR (Ab-3), mouse monoclonal, clone 225 recognizes the ~170 kDa epidermal growth factor receptor protein. It is validated for use in immunoprecipitation and neutralization.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa epidermal growth factor receptor protein.
Recognizes the ~170 kDa epidermal growth factor receptor protein in A431 cells.
Immunogen
Human
partially purified, human EGFR from A431 cells
Application
Immunoprecipitation (1-2 µg per sample)
Neutralization Studies (2 µg/ml, see comments and application references)

Physical form
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA.
Preparation Note
Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate at 4°C with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.
Analysis Note
Negative Control
HL-60 cells
Positive Control
A431 cells
Other Notes
Hunter T. 1984. Nature311, 414.
Reynolds, F.H., et. al 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody has been shown to inhibit human tumor xenographs in nude mice. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. It also inhibits A431 tumor formation in nude mice. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)