| UNSPSC Code | 12352207 |
|---|---|
| Web Name Suffix Override | lyophilized powder, suitable for hybridoma, BioXtra |
| NACRES | NA.75 |
| sterility | γ-irradiated |
| product line | BioXtra |
| form | lyophilized powder |
| technique(s) | cell culture | hybridoma: suitable |
| impurities | endotoxin, tested |
| web metadata keyword.default | hypoxanthine-thymidine supplement, hybridoma cloning, hybridoma selection |
| storage temp. | −20°C |
| Quality Level | 200 |
Answer:No. HT contains hypoxanthine and thymidine but omits the aminopterin present in HAT supplement.
Answer:Reconstitute one vial with 10 mL of sterile cell culture medium to form 50× stock, then dilute to prepare 500 mL of 1× medium.
Answer:It is commonly used during culture after HAT selection when the selective inhibitor aminopterin is no longer required.
Sigma-Aldrich Hybri-Max HT Media Supplement provides hypoxanthine and thymidine without aminopterin for hybridoma culture and related mammalian cell culture applications. The freeze-dried, gamma-irradiated BioXtra supplement is supplied as ten vials of 50× medium additive. Each vial can be reconstituted with 10 mL of sterile cell culture medium, yielding enough stock to prepare 500 mL of 1× supplemented culture medium. At final working concentration, HT provides 100 µM hypoxanthine and 16 µM thymidine. By omitting aminopterin, the supplement differs functionally from HAT medium used during initial hybridoma selection. It can support culture following removal of HAT selection pressure in workflows where hypoxanthine and thymidine remain beneficial. Culture medium formulation, timing of supplementation and appropriate serum conditions depend on the cell line. The lyophilized supplement is not a complete basal medium and requires preparation before addition to a suitable cell culture formulation.
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