Description
General description
HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Application
HEPES buffer finds application in: Most studies with metal ions
Substitute for Tris and phosphate in studies with metal ions
Grinding buffer in plant studies
Running buffer in gel electrophoresis
Buffer in electroporation studies
Bradford or bicinchoninic acid (BCA) assays
Extraction buffer to prevent damage of proteins in red blood cells
It has been used:as a buffer component in the preparation of a low sucrose buffer
as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
Features and Benefits
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Highly soluble in water
Negligible metal ion binding
Less toxic to cells than other buffers such as Tris and phosphate
Stable in a wide pH range
Packaging
The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR® packaging:The current DRYPOUR® packaging configuration is in a 50L nestable drum
25KG-DP are the standard prepack offering
Smaller volumes can be put in the same drum for variable configurations
The benefits of DRYPOUR®:Improves operational efficiency by reducing material preparation time and increasing operator safety
Minimizes caking of hygroscopic salts
Offers triple protection: against moisture inside and outside, and against contamination
How does the DRYPOUR®packaging system Improve your operational efficiency?This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek® liner
Desiccant bags are firmly integrated into the polyethylene liner
Triple protection delivers two invaluable results:
Dramatically reduced caking
No contamination risk from the desiccant
Physical form
Lyophilized, spray dried, or crystallized material.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company
