Description
General description
Cells release extracellular vesicles (EV), including exosomes, as part of normal physiological processes including removal of waste and regulation of intercellular communication. Furthermore, increased exosome release has been implicated in the progression of various disease states and other abnormalities such as cancer and neurodegeneration. Exosome identification, classification, and differentiation from other extracellular vesicles is often complicated due to the variability in isolation and preparation protocols, exosome biogenesis, as well as exosome source. Commonly associated exosome-enriched markers include tetraspanins CD9, CD63, and CD81, cargo sorting-associated markers TSG101 and Flotillin-1, and biogenesis marker syntenin-1. Other markers, including Ago2, GAPDH, and Calreticulin, may or may not be found in exosome-enriched samples, but this can vary depending on source, isolation method, and/or EV biogenesis mechanism.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 9 analytes in exosomes isolated from serum, plasma, and tissue culture supernatants.
Analytes included: Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101
Note: The Ago2 bead is static and will always be included in the kit format.
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
Comprehensive Marker Coverage: This panel includes a range of markers associated with exosome biogenesis, cargo sorting, and general ceullar function. This allows for detailed characterization of exosomes, providing insights into their biological roles and implications in various diseases.
Quantitative and Qualitative Analysis: This dual capability allows researchers to assess both the presence and concentration of specific exosomal markers providing a comprehesive understanding of exosomal profiles.
Flexibility in Exosomal Isolation Methods: This kit allows users to employ various exosome isolation methods according to their laboratory preferences and established protocols.
Configurable Kit: Design your multiplex kit by selecting from a variety of available analytes, tailoring assays to meet your specific research needs.
Control Included: The MCF7:IGF-1 Cell Lysate is included as a control for the qualitative assay of Argonaute-2. This control ensures the reliability of the qualitative results, helping to verify experimental conditions and support data interpretation.
Flexibility in Lysis Buffer Selection: Researchers can use their preferred lysis buffer, with recommendations provided for Cell Signaling Lysis Buffer.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
