Merck, HT501850-200L, Formalin solution, neutral buffered, 10%

General description
10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
Application
Popular general purpose histological fixative.
Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.

• It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
• It may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
• It is used for routine histological staining, providing clear and detailed images of cellular structures.

Biochem/physiol Actions
Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.
Features and Benefits

• 10% NBF is well-suited for large throughput laboratories.
• It requires a relatively short period of fixation.
• It can be used for the long-term storage of tissue.

Physical form
Neutral buffered solution