General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Melanin-concentrating hormone (MCH) is a cyclic, 19 amino acid peptide produced in the lateral hypothalamus and other regions of the mammalian brain (Bittencourt et al., 1992). When administered intracerebrally or overexpressed transgenically in rodents, MCH stimulates food intake and weight gain (Ludwig et al., 2001). Two G protein-coupled receptors for MCH, MCH1 and MCH2, have been identified in humans, although rodents appear only to have MCH1 (Tan et al., 2002). Both MCH1 and MCH2 are expressed primarily in brain (An et al., 2001). MCH1 has been demonstrated by genetic and pharmacological methods in rodent models to mediate obesity caused by a high fat diet or leptin deficiency (Borowsky et al., 2002; Segal-Lieberman, et al., 2003; Marsh et al., 2002). However, MCH2 might play a similar role in the species that express it. Millipore′s cloned human MCH2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MCH2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MCH2.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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