General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The orexin/hypocretin peptides orexin A and orexin B (also known as hcrt-1 and hcrt-2) are 33- and 28-amino acid peptides, respectively, that are preferentially expressed in hypothalamus. The orexins bind to two GPCRs, OX1 and OX2, to increase intracellular calcium. Orexins function to increase wakefulness, primarily through OX2, which has been shown to harbor the causative mutation in narcoleptic dogs (Lin et al., 1999). In addition, the orexinergic system plays a role in feeding and stress responses (Kukkonen et al., 2002). Millipore′s cloned human OX2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OX2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at OX2 Receptor.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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