General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
PRP, encoded by the GPR10 (or hGR3) gene, is a Gq-coupled receptor for prolactin-releasing peptide that is expressed in the pituitary (Hinuma et al., 1998). Genetic studies in rodents indicate that lack of GPR10 leads to hyperphagia, obesity and dyslipidemia (Gu et al., 2004; Watanabe et al., 2005). In humans, genetic variations in GPR10 are associated with lowered blood pressure (Bhattacharyya et al., 2003). Millipore′s cloned human PRP-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PRP expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at PRP.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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