General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
MRGPRX2 (MrGX2) is a Gq- and Gi-coupled GPCR that binds to several peptides, including the neuropeptide cortistatin-14 and the proadrenomedullin N-terminal peptide (PAMP). Dorsal root ganglia and adrenal chromaffin cells express MRGPRX2, and binding of these ligands to MrgX2 is thought to play a role in nociception and catecholamine release from the adrenal glands (Robas et al., 2003; Kamohara et al., 2005). Millipore′s cloned human MRGPRX2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MRGPRX2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at MRGPRX2.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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