General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Calcium homeostasis in vertebrates is regulated by hormonal communication between the parathyroid and thyroid glands, kidney, bone, and intestine. A central mediator of this regulatory system is the calcium sensor CaS (also known as CaR), a class III GPCR that is activated by high concentrations of extracellular calcium and other divalent cations. The cell types that are responsive to variations in blood calcium concentration express CaS and respond to changes in extracellular calcium in a CaS-dependent fashion. Activation of CaS in parathyroid chief cells inhibits PTH release, which results in decreased calcium mobilization from bone. In contrast, stimulation of CaS activity in the C cells of the thyroid increases secretion of calcitonin, which causes decreased bone resorption and increased urinary excretion of calcium. Allosteric "calcimimetic" potentiators are being evaluated for treatment of primary hyperparathyroidism (Brown and MacLeod, 2001). Millipore′s cloned human CaS-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CaS expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CaS Receptor.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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