General descriptionMillipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Glucagon-like peptide 2 (GLP-2) is a 33 amino acid peptide secreted by intestinal endocrine L cells in response to nutrient uptake. GLP-2 exerts multiple effects on intestinal physiology, including promotion of epithelial cell growth and survival to increase intestinal mass, increasing nutrient absorption capacity, and inhibition of intestinal inflammation (Dubé and Brubaker, 2007). The GLP-2 Receptor is a class B GPCR belonging to the glucagon/secretin family of receptors, and as with the other members of the family, activation of the GLP-2 receptor results in increased intracellular cAMP (Munroe et al., 1999). Millipore′s cloned human GLP-2 -expressing cell line is made in the Chem-11 host, which supports high levels of recombinant GLP-2 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at GLP-2.
Other NotesPack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
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